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OperonBiology
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Cutting a plasmid twice

Topic: Biotechnology

A student digests a 5,400 bp circular plasmid with EcoRI and BamHI together, then runs the fragments on an agarose gel beside a DNA ladder with bands at 5,000, 4,000, 3,000, 2,500, 2,000, 1,500, 1,000, 750, 500 and 250 bp. The wells are at the negative electrode. Positions on the plasmid map are numbered from 0 to 5,400 bp around the circle.

Data

Restriction map of the plasmid (each enzyme cuts only at the sites listed)
EnzymeCut site position (bp)
EcoRI400
BamHI1,300
BamHI3,900

Which band pattern should the double digest give, listed from the wells downwards?

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