Prediction gridDifficulty 4/5 · HardOlympiadArgumentationVisual representation
Merodiploids: who acts in trans?
Topic: Operons: the lac operon
Jacob and Monod made partial diploids of E. coli: a cell with its own chromosome plus an F′ plasmid carrying a second copy of the lac region. Glucose is absent throughout. β-galactosidase activity is measured with and without an inducer (IPTG).
Predict β-galactosidase activity for each strain, with and without inducer.
I⁺ O⁺ Z⁺ (wild type)
No inducerWith inducerI⁻ O⁺ Z⁺
No inducerWith inducerI⁻ O⁺ Z⁺ / F′ I⁺ O⁺ Z⁺
No inducerWith inducerIˢ O⁺ Z⁺ / F′ I⁺ O⁺ Z⁺
No inducerWith inducerI⁺ Oᶜ Z⁺ / F′ I⁺ O⁺ Z⁻
No inducerWith inducerI⁺ Oᶜ Z⁻ / F′ I⁺ O⁺ Z⁺
No inducerWith inducer
| Row | No inducer | With inducer |
|---|---|---|
| I⁺ O⁺ Z⁺ (wild type) | ||
| I⁻ O⁺ Z⁺ | ||
| I⁻ O⁺ Z⁺ / F′ I⁺ O⁺ Z⁺ | ||
| Iˢ O⁺ Z⁺ / F′ I⁺ O⁺ Z⁺ | ||
| I⁺ Oᶜ Z⁺ / F′ I⁺ O⁺ Z⁻ | ||
| I⁺ Oᶜ Z⁻ / F′ I⁺ O⁺ Z⁺ |
Answer every part to submit.